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The effect of interleukin‐1 β on hyaluronic acid synthesized by adult human gingival fibroblasts in vitro
Author(s) -
Bartold P. Mark
Publication year - 1988
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1988.tb01347.x
Subject(s) - hyaluronic acid , cycloheximide , chemistry , biochemistry , cell growth , hyaluronan synthase , fibroblast , in vitro , microbiology and biotechnology , pharmacology , protein biosynthesis , biology , genetics
The effect of recombinant interleukin‐1β (IL‐lβ) on hyaluronic acid synthesis by human gingival fibroblasts was studied. IL‐1bT caused a dose‐dependent increase in the incorporation of ( 3 lucosamine into hyaluronic acid. The 35 S/35H ratios of labeled macromolecules did not change regardless of the presence or absence of TL‐lβ and indicates stimulation of hyaluronic acid synthesis. Inhibition of cell proliferation by hydroxyurea caused an increase in hyaluronic acid synthesis. The effect of IL‐1β on hyaluronic acid synthesis in the presence of hydroxyurea was increased over untreated and IL‐lβ‐treated controls, but equivalent to the hydroxyurea‐treated controls. Thus the effect of IL‐1β on hyaluronic acid synthesis may be independent of cell proliferation. Furthermore, inhibition of prostaglandin E2 synthesis by indomethacin abolished the effect of IL‐1β on hyaluronic acid synthesis. Inhibition of new protein synthesis by cycloheximide negated the effect of IL‐β on hyaluronic acid synthesis. This may be related to inhibition of new hyaluronate synthetase synthesis, since IL‐1β stimulated the level of hyaluronate synthetase activity. Sepharose CL‐2B chromatography revealed that most of the newly synthesized hyaluronic acid was of large molecular size. The cells exposed to IL‐1β retained more large molecular size hyaluronic acid in their cell layer environment than did the control cells. These responses by fibroblasts to IL‐1β may be indicative of early tissue repair.