Premium
Serological classification of Bacteroides from the human oral cavity
Author(s) -
Ebersole J. L.,
Taubman M. A.,
Smith D. J.,
Frey Deirdre E.
Publication year - 1988
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1988.tb01022.x
Subject(s) - bacteroides , microbiology and biotechnology , antigen , biology , bacteroidaceae , periodontal disease , serology , antiserum , homology (biology) , antibody , immunology , medicine , bacteria , genetics , gene
Studies were initiated to investigate the antigenic relationship among oral species of Bacteroides . Antigenic differences among the Bacteroides were assessed with an ELISA using antisera from immunized rabbits and sera obtained from subjects with periodontal disease. Multiple species of Bacteroides were examined, including: B. gingivalis , 2 homology groups of B. intermedius, B. melaninogenicus, B. denticola, B. loescheii, B. oralis, B. levii, B. gracilis, B. buccae. and B. corporis. Both rabbit and human sera reacted with distinct surface antigenic determinants in each of the species of Bacteroides. Additional relationships were defined, such that the 2 homology groups of B. intermedius exhibited a similarity of approximately 30–40%, while the B. melaninogenicus and B. loescheii showed a 20–25% similarity of surface antigens. B. denticola was antigenically related to the B. intermedius and B. melaninogenicus strains by 10–15%. The remaining cross‐reactivities were generally less than 5% among the Bacteroides species. The findings are consistent with specific surface antigenic determinants on the oral Bacteroides spp. and suggest the ability to detect specific responses to these organisms in periodontal disease patients.