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Collagenolytic activity in crevicular fluid from patients with chronic adult periodontitis, localized juvenile periodonitis and gingivitis, and from healthy control subjects
Author(s) -
Villela Benardo,
Cogen Ronald B.,
Bartolucci Alfred A.,
BirkedalHansen Henning
Publication year - 1987
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1987.tb01603.x
Subject(s) - gingivitis , collagenase , periodontitis , medicine , chronic periodontitis , periodontal disease , dentistry , gastroenterology , enzyme , biology , biochemistry
Collagenolytic activity in gingival crevicular fluid (GCF) sampled from 25 healthy control subjects, 25 gingivitis, 25 chronic adult periodontitis (CAP) and 8 LJP patients was correlated with clinical disease parameters using both the site and the patient as sampling units. Among patients collagenase activity increased with the severity of the disease in the order: healthy < gingivitis < periodontitis. Among sites , significant correlation was found between GCF collagenase activity and pocket depth in CAP and LJP, but not in gingivitis patients. Enzyme activity was also correlated with GI score in LJP, but not in CAP and gingivitis patients. In a subset of 10 patients in each of the healthy, gingivitis and CAP groups the association of enzyme activity and crevicular fluid volume (flow) was examined. Significant correlation was found between fluid volume and pocket depth in CAP patients, and between fluid volume and GI score in gingivitis patients, but no association was observed between collagenase activity and fluid volume. The Collagenolytic enzyme was shown to be a genuine vertebrate collagenase derived from unidentified host cells. The concentration of the enzyme in crevicuar fluid from CAP patients was in the order of 10 μg/ml.