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Collagenolytic activity of black‐pigmented Bacteroides species
Author(s) -
Sundqvist G.,
Carlsson J.,
Hänström L.
Publication year - 1987
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1987.tb01589.x
Subject(s) - dithiothreitol , chemistry , bacteroides , hydrogen peroxide , chelation , zinc , egta , thiol , bacteroidaceae , biochemistry , reagent , enzyme , bacteria , biology , calcium , inorganic chemistry , organic chemistry , genetics
Strains of black‐pigmented Bacteroides were examined for their ability to degrade collagen, albumin and benzoyl‐D‐L‐arginine‐p‐nitranilide (BAPNA). B. gingivalis was the only species that showed activity against collagen and BAPNA. This activity was cell‐bound, stabilized by dithiothreitol and inhibited by sulphy‐dryl‐modifying reagents such as mercuric, cupric and zinc ions, 5.5′‐dithiobis‐2‐nitrobenzoic acid, p‐chloromercuriphenylsulfonic acid and hydrogen peroxide. The activity was also inhibited by the chelating agents EDTA and EGTA. This indicated that B. gingivalis has cell‐bound thiol proteinase activity dependent on metal ions.