Premium
Collagenase and collagenase inhibitor activities in crevicular fluid of patients receiving treatment for localized juvenile periodontitis
Author(s) -
Larivée Jacinthe,
Sodek and Jaro,
Ferrier Jack M.
Publication year - 1986
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1986.tb01508.x
Subject(s) - collagenase , periodontitis , medicine , interstitial collagenase , inflammation , pathology , chemistry , endocrinology , enzyme , biochemistry
Collagenolytic activity was monitored in crevicular fluids of 3 patients receiving treatment for localized juvenile periodontitis (LJP) and in 3 control, clinically healthy patients to study relationships between mammalian collagenase and disease activity. It was found that tissue collagenase activity initially was significantly higher in diseased patients compared to controls, but that in II of 13 diseased sites the activity decreased substantially following clinical treatment. In control sites, active collagenase was generally low or absent. Latent collagenase concentrations tended to remain high in diseased sites and in sites with gingival inflammation. Collagenase inhibitor activity was found only in clinically healthy sites sampled from both control subjects and LJP patients throughout the experiment, and in diseased sites following various phases of treatment. The collagenolytic activity observed was typical of mammalian collagenase in that the primary degradation products of collagen were 3/4‐ and 1/4‐fragments. However, additional specific fragmentation of native collagen 3/4‐fragments was also observed, which could be attributed to a novel collagenolytic activity generally present in crevicular fluid in a latent form. The results of this study suggest that the determination of active collagenase and collagenase inhibitor in crevicular fluid might be useful in assessing both the disease status of periodontal tissues and the efficacy of clinical treatment.