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Immunohistochemical investigation of the presence and distribution of cementum‐associated lipopolysaccharides in periodontal disease
Author(s) -
Hughes F. J.,
Smales F. C.
Publication year - 1986
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1986.tb01503.x
Subject(s) - cementum , dental cementum , antiserum , chemistry , periodontal disease , immunohistochemistry , connective tissue , demineralization , antibody , sepharose , microbiology and biotechnology , pathology , dentistry , medicine , immunology , biology , biochemistry , enzyme , enamel paint , dentin
An immunohistochemical technique was used to investigate the distribution of cementum‐associated LPS in 14 human teeth extracted because of periodontal disease. Autologous affinity purified anti‐LPS antibodies were obtained against a preparation of oral LPS types bound to an epoxy‐activated Sepharose 6B‐polymyxin B column. The concentration of immunoglobulin classes G. M and A in the prepared sera was assayed by rocket immunoelectrophoresis and the reactivity of the sera was checked using an ELISA to the LPS preparation. Ground sections of the teeth were prepared and incubated with prepared autologous antiserum, followed by detection of Ig binding by a rabbit PAP system. LPS was detected on cementum which had been previously exposed to a periodontal pocket and extended to within I mm of the previous level of connective tissue attachment. LPS was only detected on the surface of cementum and on no occasion was LPS seen penetrating into sub‐surface cementum. These results suggest that extensive removal of sub‐surface cementum is not necessary in order to render root surfaces free of bacterial endotoxins during root planing procedures.