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Human complement activation by purified Capnocytophaga exopolysaccharide. Measurement by radioimmunoassay
Author(s) -
Bolton R. W.,
Dyer J. K.
Publication year - 1986
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1986.tb01500.x
Subject(s) - radioimmunoassay , egta , complement system , alternative complement pathway , capnocytophaga , classical complement pathway , chemistry , biochemistry , chelation , antibody , chromatography , microbiology and biotechnology , bacteria , biology , immunology , calcium , genetics , organic chemistry
Exopolysaccharide (EP) purified from spent culture fluid of three Capnocytophaga species was examined for its ability to activate the human complement system. Heparinized plasma was absorbed with bacterial cells to remove natural antibody. Aliquots of plasma were then incubated with EP for 1 h at 37 C and complement activation was evaluated by a radioimmunoassay specific for C3a (Upjohn Diagnostics). EP from each species of Capnocytophaga activated the complement system. The amount of C3a generated was dose‐dependent over a range from 1 to 1000 μg EP/ml plasma and reached a maximum of 28 μg C3a/ml plasma. Chelation of Ca ++ with EGTA inhibited complement activation by EP, which suggested that activation occurred by the classical pathway.