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Saliva and salivary sulphated glycoprotein inhibit adhesion and locomotion of human gingival fibroblast‐like cells in vitro
Author(s) -
Heaney T. G.,
Embery G.,
Green D.
Publication year - 1986
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1986.tb01459.x
Subject(s) - saliva , fibroblast , in vitro , glycoprotein , saline , microbiology and biotechnology , chemistry , adhesion , andrology , immunology , biology , biochemistry , endocrinology , medicine , organic chemistry
The purpose of this study was to determine the influence of clarified human whole saliva on the attachment and locomotion of human gingival fibroblast‐like cells to plastic substrata in vitro. Saliva was subjected to gel filtration on Sepharose CLAB to isolate a high molecular weight sulphated glycoprotein fraction (SGP, Hogg & Embery 1979, 1982) from the residual fractions (RSF) which were pooled. 25 cm 2 plastic culture dishes were coated with either sterile whole saliva, SGP (0.3 mg/ml) or RSF (0.6 mg/ml) in Ringer‐Locke saline. They were then seeded with 2.50 × 10 3 fibroblasts suspended in Ringer‐Locke saline and numbers of adhering cells determined at times up to 60 min.Cell adherence in both saliva and glycoprotein‐treated dishes was significantly less than that in untreated control dishes (p < 0.01, p < 0.05, respectively), but RSF treatment had no effect on attachment. Incubating the cells with saliva or SGP prior to plating did not significantly suppress their subsequent attachment behavior when cell death caused by the incubation procedure was taken into account. Migration of fibroblasts from confluent monolayers on glass coverslips onto the surface of SGP‐ or saliva‐treated dishes was significantly delayed compared with controls, for periods up to 5 d (p < 0.01, p < 0.0 respectively). It was concluded that whole saliva inhibits the attachment or locomotion of gingival fibroblast‐like cells on plastic substrata in vitro, and that this property is primarily mediated by its glycoprotein component.