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Radioautographic analysis of 3 H‐fucose utilization by fibroblasts of the periodontal ligament
Author(s) -
Cho MoonIL,
Garant Philias R.
Publication year - 1986
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1986.tb01439.x
Subject(s) - periodontal fiber , fucose , extracellular matrix , glycoprotein , secretion , golgi apparatus , extracellular , chemistry , matrix (chemical analysis) , ligament , microbiology and biotechnology , fibroblast , cell , biochemistry , anatomy , biology , dentistry , medicine , in vitro , chromatography
The secretion of 3 labeld glycoproteins from periodontal ligament fibroblasts was, studied by light and electron microseopic radioautography. At 5, 10. and 20 minutes after intravenous injection, 3 H‐fucose was concentrated in Golgi cisternae and saccules. By 35 minutes, the label was dispersed to the cell periphery and extracellular matrix. At 8 hours after injection, almost all of the radioactive label was associated with the cell surface or the adjacent extracellular matrix. Labeled glycoprotein was contained in collagen secretion granules and appeared to be relesasd simultaneousley with the collagen precursors The distribution of 3 H‐fucose labeled material was uniform across the periodontal ligament.