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Histochemical demonstration of lectinbinding sites and keratin in inflamed human gingiva
Author(s) -
Murase N.,
Hosaka M.,
Takai Y.,
Tanimura T.,
Mori M.
Publication year - 1985
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1985.tb00847.x
Subject(s) - keratin , staining , lectin , chemistry , glycosaminoglycan , cell , galactosamine , sudan black b , microbiology and biotechnology , biology , biochemistry , glucosamine , paleontology , genetics
Histochemical demonstration of lectin‐binding sites and keratin peptides in gingival epithelia was reported and differences in staining and distribution were compared to inner and outer gingival epithelia. Gingival epithelia on the outer side exhibited zonal or regional distributions of lectin‐binding, and the cytochemical staining was generally found in the cell coat and intercellular materials. Keratin protein was found frequently in the spinous cell, infrequently in the basal cells, and not at all in the superficial layer. The sugar residues in the cell coat of gingival epithelia probably were mannose, galactose, N‐acetyl‐galactosamine, and N‐acetyl‐glucosamine. The inner side of the epithelia, crevicular, and pocket epithelia were characterized by irregular and incomplete staining for lectins. These epithelia also displayed less keratin staining compared to the outer gingival epithelia. These findings suggest the possibility of depolymerization of glycosaminoglycans in the cell coat by enzymes of either pocket bacterial origin or host tissue origin.