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Comparative study of six random oral spirochete isolates
Author(s) -
Cheng S.L.,
Siboo R.,
Chin Quee T.,
Johnson J. L.,
Mayberry W. R.,
Chan E. C. S.
Publication year - 1985
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1985.tb00844.x
Subject(s) - treponema denticola , microbiology and biotechnology , treponema , fastidious organism , serotype , biology , homology (biology) , agglutination (biology) , serology , bacteria , virology , antigen , antibody , genetics , porphyromonas gingivalis , amino acid , syphilis , human immunodeficiency virus (hiv)
Six anaerobic oral spirochetes designated strains a, b, c, d, e, and e’ were isolated randomly from periodontal pockets and were maintained in pure culture. The strains were found to belong to the genus Treponema . All strains were similar in cultural, morphological, biochemical, and physiological properties with the exception of the following features: strains c and d were more fastidious nutritionally for sodium bicarbonate as a medium supplement than the other strains; they differed in the number of axial fibrils; and they were also serologically distinct from strains a, b, e, and e’ by agglutination tests and fluorescent immunoassays. However, DNA homology studies indicated that all the isolates were strains of Treponema denticola with divergent phenotypic and serological properties. Serovar a and serovar c, representing two different serogroups, have been deposited in the American Type Culture Collection with the accession numbers ATCC 35405 and ATCC 35404, respectively.