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The quantification of individual proteins in crevicular gingival fluid
Author(s) -
Marcus E. R.,
Jooste C. P.,
Driver H. S.,
Hattingh J.
Publication year - 1985
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1985.tb00826.x
Subject(s) - transudate , radial immunodiffusion , albumin , fibrinogen , chemistry , immunoelectrophoresis , pathology , inflammation , tissue fluid , medicine , immunology , antibody , biochemistry , pleural fluid , pleural effusion
The aim of the present study was the quantification of proteins present in crevicular fluid obtained from individual sulci, and to this effect simplified micromethods for the quantification of individual proteins were developed. Unstimulated, resting crevicular fluid from healthy crevicular spaces (i.e. in the absence of clinically detectable inflammation) of dogs and humans was collected for 3 minutes, either in microcapillary glass tubes or with the aid of filter paper strips. Quantification of proteins was done by radial immunodiffusion without the pooling of samples or by immunoelectrophoresis, without eluting from the papers. The concentrations of albumin and fibrinogen in crevicular fluid so determined proved to be significantly lower than plasma concentrations. These results strengthen the concept that capillary dynamics in healthy gingivae are similar to those in general tissue capillary beds and that gingival fluid is a plasma transudate. However, due to ever present sub‐clinical inflammation and other factors, it is improbable that fluid from even healthy sulci is a true transudate.