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Binding of 3 H‐Methyltrienolone ( 3 H‐R1881) to androgen receptors in human gingiva
Author(s) -
Vittek J.,
Hernandez M. R.,
Wenk E. J.,
Rappaport S. C.,
Southren A. L.
Publication year - 1985
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1985.tb00409.x
Subject(s) - androgen receptor , receptor , chemistry , pronase , androgen , biochemistry , proteolytic enzymes , binding site , trypsin , enzyme , endocrinology , medicine , biology , hormone , prostate cancer , cancer
Binding of 3 H‐Methyltrienolone ( 3 H‐R1881) to cytoplasmic androgen receptor protein and its translocation into nuclei in human gingiva was demonstrated. 3 H‐R1881 binds to androgen receptors with high affinity (Kd= 1.7−1.9 × 10 −9 M) and low capacity (10–16 fmol/mg protein). The binding was specific for DHT binding sites and was destroyed by proteolytic enzymes (pronase and trypsin). These results suggest that 3 H‐R1881 can be used as a ligand in combination with nonradioactive DHT to study androgen receptors in the gingiva.