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Measurement of eight prostaglandins in human gingival and periodontal disease using high pressure liquid chromatography and radioimmunoassay
Author(s) -
Ohm K.,
Albers H.K.,
Lisboa B. P.
Publication year - 1984
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1984.tb01305.x
Subject(s) - radioimmunoassay , high performance liquid chromatography , prostaglandin e , thromboxane , chemistry , prostaglandin , prostacyclin , thromboxane b2 , gingivitis , prostaglandins f , medicine , endocrinology , chromatography , biochemistry , dentistry , platelet
Eight prostaglandins (PG) of biological importance, namely PGE,. PGE 1 13,14‐dihydro‐15‐keto‐PGE, (DHK‐PGE 2 ), PGF 2α , PGF 1α , 13, 14‐dihydro‐15‐keto‐PGF 2α (DHK‐PGF 2α , 6‐keto‐PGF 1α , and thromboxane B 2 (TxB 2 ), were measured in the human gingiva in a healthy state, gingivitis. and periodontitis. High pressure liquid chromatography (HPLC) in combination with radioimmunoassay (RIA) was employed for quantitative analysis. The advantages of HPLC over the classic silicic acid column chromatography of prostaglandins were discussed. All prostaglandins considered were shown to increase during advancing periodontal destruction. The most quantitatively significant endoperoxide metabolites at every stage of inflammation were TxB 2 and 6‐keto‐PGF 1α followed by PGE 2 . DHIK‐PGE 2 , PGE 1 , PGF 1α. PGF 3α , and DHK‐PGF 2α in decreasing order.