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Proteoglycan production by chick embryonic chondrocytes is inhibited by culture filtrate of Bacteroides gingivalis
Author(s) -
Kampen G. P. J.,
Steenbergen T. J. M.,
Schipper C. A.,
Graaff J.,
Veldhuijzen J. P.
Publication year - 1984
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1984.tb01303.x
Subject(s) - proteoglycan , in vitro , bacteroides , chemistry , biochemistry , cell culture , sulfation , microbiology and biotechnology , bacteria , biology , extracellular matrix , genetics
Culture filtrate of Bacteroides gingivalis was tested in vitro for its effect on the proteoglycan synthesis ( 35 S‐sulfate incorporation) of chick embryonic chondrocytes. The chondrocytes were cultured in high density aggregates. The culture medium (M 199 with 10% fetal calf serum) was supplemented with 20% culture filtrate, 20% heat inactivated culture filtrate (100°C, 10 min), or 20% of a low molecular fraction (M < 1000) of the culture filtrate. Control aggregates were cultured with 20% bacterial medium added to the culture medium. Exposure of chondrocytes to culture filtrate resulted in a rapid reduction of the synthesis of proteoglycans within 5 hours. After 24 hours of culture proteoglycan synthesis was decreased by 80%. The small amount of proteoglycans still synthesized was mainly deposited into the medium whereas in control aggregates most proteoglycans were deposited around the cells. Protein synthesis was not changed and DNA synthesis was slightly increased by the culture filtrate. When after 24 hours of culture the medium with culture filtrate was replaced by control medium, this resulted in a rapid and total repair of the proteoglycan synthesis. The effect of culture filtrate on the proteoglycan synthesis did not change after heat inactivation or using the low molecular fraction, indicating that small, non‐proteinous molecules are responsible for the described effects.