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Evaluation of serum‐containing substrates for cultivation of oral spirochetes
Author(s) -
Fiehn N.E.,
Frandsen A.
Publication year - 1984
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1984.tb01196.x
Subject(s) - nalidixic acid , antibiotics , microbiology and biotechnology , rifampicin , bacteria , polymyxin b , treponema , polymyxin , periodontitis , biology , chemistry , medicine , dentistry , ciprofloxacin , immunology , genetics , syphilis , human immunodeficiency virus (hiv)
The aim of this study was to evaluate eight different solid substrates with rabbit serum and ±5% blood for suitability to support growth of oral spirochetes in subgingival plaque samples from patients with advanced destructive periodontitis. In order to inhibit growth of other bacteria, nine antibiotics were added to the substrates singly or in combinations. Four of the tested substrates supported growth of oral spirochetes, BHIA‐SC and GM‐1 (both with blood), yielding the best results with a recovery of about 4%. The antibiotics best suited for the suppression of other bacteria were rifampicin, polymyxin B, and nalidixic acid.