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Metabolic change in cultured gingival fibroblasts exposed to bacterial extracts
Author(s) -
Larjava Hannu
Publication year - 1984
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1984.tb00814.x
Subject(s) - hyaluronic acid , cycloheximide , stimulation , connective tissue , lipopolysaccharide , chemistry , fibroblast , microbiology and biotechnology , bacteria , biochemistry , dental plaque , bacillus cereus , biology , protein biosynthesis , endocrinology , medicine , pathology , in vitro , genetics
Hyaluronic acid synthesis was studied in human gingival fibroblast cultures exposed to bacterial extracts from dental plaque, Bacillus cereus , and Escherichia coli (lipopolysaccharide, LPS). Dental plaque extract was the best stimulant of hyaluronic acid synthesis. B. cereus extract was almost as effective, but the effect of LPS was insubstantial. This indicates that gram‐positive microorganisms of dental plaque, as well as gram‐negative bacteria, may be involved in the stimulation of hyaluronic acid synthesis in gingival fibroblasts. The effect of indomethacin, cortisol, and cycloheximide on hyaluronic acid synthesis were studied in control and plaque extract treated cultures. Indomethacin had no effect whereas cortisol inhibited the synthesis both in control and treated cultures. Cycloheximide prevented the stimulation of hyaluronic acid synthesis in treated cultures. This suggests that protein synthesis is required for the stimulation of hyaluronic acid synthesis in the presence of plaque extract. The altered metabolism of connective tissue cells due to dental plaque may be important in the development of periodontal disease.