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Cell migration in the periodontal ligament of mice
Author(s) -
McCulloch C. A. G.,
Melcher A. H.
Publication year - 1983
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1983.tb00369.x
Subject(s) - periodontal fiber , cementum , labelling , dental alveolus , population , cell , molar , chemistry , periodontitis , andrology , pathology , biology , anatomy , medicine , dentistry , dentin , biochemistry , environmental health
Cell migration and the rate at which cells divide was examined in the periodontal ligament of mouse molars. A pulse injection of 3 H‐Tdr was administered, and mice were sacrificed between 1 hour and 60 days after injection. Analysis of radioautographs using labelling index and grain counts demonstrated that the majority of labelled cells divided within 3 days but a measurable population of cells had not divided after 14 days. These cells were G 2 ‐blocked. The labelling index of cells adjacent to alveolar bone increased 8 times within 1 day, indicating that labelled cells had migrated to the bone surface. Migration of cells to the vicinity of the cementum was observed 3 days after labelling. The percentage of labelled cells located within 20μm of one another increased to 40% within 3 days, suggesting clonal proliferation of periodontal ligament cells.