Premium
An attempt to simulate junctional epithelium of human gingiva in vitro
Author(s) -
Salonen J.,
Santti R.
Publication year - 1983
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1983.tb00365.x
Subject(s) - explant culture , hemidesmosome , basal lamina , junctional epithelium , epithelium , organ culture , matrix (chemical analysis) , anatomy , microbiology and biotechnology , biology , connective tissue , in vitro , pathology , chemistry , ultrastructure , medicine , biochemistry , chromatography
Pieces of human masticator mucosa were maintained in a Trowell‐type organ culture. The explants were placed on their side on either microporous filters or decalcified dentin matrix slices. The presence of serum in the culture medium enhanced epithelial outgrowth (epibolus) from the explants. The epibolus grew longer in contact with the substratum than along the free connective tissue surface of the explant. When grown on microporous filters, the flow of migrating cells was directed between the explant and substratum. As a sign of epithelial attachment to the filter, a structure morphologically similar to basal lamina and hemidesmosomes was seen. The decalcified dentin matrix, however, caused the epithelial cells to migrate outwards from the explant. The explants became loosely attached to the matrix. Although some electron‐dense material was secreted into the contact area, a typical basal lamina was not seen.