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Con A stimulation of collagenase synthesis by human gingival fibroblasts
Author(s) -
Wang H.M.,
Hurum S.,
Sodek J.
Publication year - 1983
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1983.tb00347.x
Subject(s) - collagenase , stimulation , wheat germ agglutinin , incubation , chemistry , pokeweed mitogen , secretion , biochemistry , fibroblast , enzyme , trypsin , medicine , endocrinology , microbiology and biotechnology , biology , concanavalin a , lectin , in vitro
Presence of 10–1000 μg/ml of Con A in cultures of human gingival fibroblasts promoted the synthesis and secretion of collagenase. Incubation with Con A for a minimum of 6 hours was required for the effect which could be inhibited over this time period by the addition of α‐methyl mannoside. In the continuous presence of Con A, a latent period of at least 8 hours was required before the stimulating effect was observed. Con A also stimulated the synthesis of a number of other unidentified proteins secreted into the medium. Of other lectins tested, phytohemagglutinin produced a moderate stimulation of collagenase production, but only a small effect was observed with pokeweed mitogen and wheat germ agglutinin. The enzyme, which was detected almost entirely in a latent form, was shown to be a true collagenase through the production of typical 3/4‐ and 1/4‐ fragments from a collagen substrate at neutral pH and the inhibition of activity by sulfhydryl compounds and metal chelators.