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Lymphocytes from chronically inflamed human gingiva
Author(s) -
Daly C. G.,
Cripps A. W.,
Clancy R. L.
Publication year - 1983
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1983.tb00345.x
Subject(s) - pokeweed mitogen , antibody , radioimmunoassay , gingivitis , immunoglobulin g , in vivo , in vitro , immunoglobulin m , immunology , immunoglobulin a , tissue culture , medicine , andrology , chemistry , biology , dentistry , peripheral blood mononuclear cell , biochemistry , microbiology and biotechnology
Immunoglobulin production in vitro was investigated for lymphocytes recovered from gingival tissue of 16 patients with chronic marginal gingivitis. Recovered lymphocytes were maintained in culture for 7 days, and the presence of IgG, IgA, and IgM in culture supernatants quantitated using solid‐phase radioimmunoassay. The production of IgG was assessed for 4 patients at days 1, 3, 5, and 7 whilst release of synthesized immunoglobulin due to repeated freeze‐thawing was investigated immediately following cell recovery in 2 patients. The effect of adding pokeweed mitogen (PWM) to gingival cultures was assessed for 8 patients. The concentrations of immunoglobulins found in culture supernatants at 7 days were IgG, 6.6 μg/ml (SD ± 5.7); IgA, 0.8 μg/ml (SD ± 1.0); and IgM, 0.4 μg/ml (SD ± 0.2). Assessment of IgG production over 7 days showed that 56% of the IgG was present by 24 h, 80% by day 3, and 86% by day 5. The amounts of IgG found following repeated freeze‐thawing accounted for 15% of the 7 day total in patient 1 and 38% in patient 2. Addition of PWM to gingival cell cultures at concentrations of 1/50 through to 1/400 had no stimulatory effect on immunoglobulin production. The results indicated that lymphocytes recovered from chronically inflamed gingival tissue secreted immunoglobulin progressively throughout a 7 day culture period. The findings also suggested that gingival B lymphocytes had been highly stimulated in vivo and were actively secreting immunoglobulin at the time of recovery from tissue.