Premium
Bacteroides gingivalis antigens and bone resorbing activity in root surface fractions of periodontally involved teeth
Author(s) -
Patters M. R.,
Landesberg R. L.,
Johansson L.A.,
Trummel and C. L.,
Robertson P. B.
Publication year - 1982
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1982.tb01138.x
Subject(s) - cementum , dentin , dental cementum , resorption , chemistry , citric acid , dentistry , calculus (dental) , bone resorption , antiserum , antigen , medicine , immunology , biochemistry
Bone resorbing activity and the presence of antigens of Bacteroides gingivalis were assessed in plaque, calculus, cementum, and dentin obtained from roots of teeth previously exposed to periodontitis. Each fraction was obtained by scaling the root surface. The fractions were extracted by stirring and sonication, and the soluble extracts centrifuged, sterilized, dialyzed, and adjusted to equivalent protein concentrations. Cementum and dentin extracts from impacted teeth were prepared similarly and served as controls. Stimulation of bone resorption by each extract was assessed in organ cultures of fetal rat bones by measurement of release of previously‐incorporated 45 Ca from the bone into the medium. In some groups of teeth, calculus and cementum were treated with citric acid prior to scaling. Citric acid washes were recovered and dialyzed. An enzyme‐linked immunosorbent assay (ELISA) was used to assess the extracts for the presence of antigens reactive with an anti‐serum to B. gingivalis . Significant stimulation of bone resorption was found in all calculus and periodontally‐involved cementum preparations. ELISA showed significant levels of B, gingivalis antigens in plaque, calculus, and cementum of periodontally‐involved teeth, but not in involved dentin nor in cementum or dentin of impacted teeth. Treatment with citric acid removed essentially all B. gingivalis antigens from cementum but not calculus. The results suggest that substances which stimulate bone resorption and substances which react with B. gingivalis antiserum are present in surface plaque, calculus, and cementum of periodontally‐involved teeth. These substances are not present in cementum and dentin of impacted teeth nor in dentin of periodontally‐involved teeth. Treatment by both scaling and citric acid demineralization will remove most of these substances from cementum of teeth previously exposed to periodontitis.