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Microbiological studies on early dento‐gingival plaque on teeth and Mylar strips in humans
Author(s) -
Theilade Else,
Theilade Jørgen,
Mikkelsen Lena
Publication year - 1982
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1982.tb01127.x
Subject(s) - veillonella , dental plaque , microbiology and biotechnology , actinomyces , coccus (insect) , dentistry , buccal administration , tooth surface , streptococcus , biology , medicine , bacteria , ecology , genetics
The predominant cutivahle flora of 3‐ and 8‐hr‐old dento‐gingival plaque formed on a buccal tooth surface and on Mylar strips ligated to a buccal surface was studied in 6 students with healthy gingiva. Differential counts of various bacterial types were also performed by direct microscopy. In 6 other students. 1‐ and 3‐day‐old plaque was similarly studied. From each sample. 31–111 strains (a total of 2,867 strains) were characterized. Streptococci predominated at all times. Most of these were Streptococcus milior. Sfreptococcus sunguis and Streptococcus milleri. Very few Gram‐negative cocci were isolated from 3‐ and 8‐hr samples, whereas Veillonella constituted about 20 Q of the strains from 1‐ and 3‐day plaque. Gram‐positive rods increased from low proportions in most 3‐ and 8‐ hr samples to median values of 21% and 25% in 1‐ and 3‐day plaque. In 3‐ and 8hr samples all the Gram‐positive rods were facultative anaerobes (most of them Actinomyces viscosus and Actinomyes nueslundii). In 1‐ and 3‐day samples obligatdy anaerobic Grampositive rods also appeared. Gram‐negative rods were virtually absent from 3‐ and 8‐hr plaque. Ohligately anaerobic Gram‐negative rods appeared in low proportions in 1‐ and 3day samples (median 4% and 7%). Large site‐to‐site variations were found but no systematic differences between samples from Mylar and enamel surfaces.