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A highly sensitive and specific assay for vertebrate collagenase
Author(s) -
Sodek J.,
Hurum S.,
Feng J.
Publication year - 1981
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1981.tb00993.x
Subject(s) - collagenase , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , chemistry , gel electrophoresis , microbiology and biotechnology , chromatography , lysis , cell culture , cell , microbial collagenase , biochemistry , enzyme , biology , genetics
A highly sensitive and specific assay for vertebrate collagenase has been developed using a [ 14 C]‐labeled collagen substrate and a combination of SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) and fluorography to identify and quantitate the digestion products. The assay was sufficiently sensitive to permit the detection and quantitation of collagenase activity in 0.1 μl of gingival sulcal fluid, and in samples of cell culture medium without prior concentration. The assay has also been used to detect the presence of inhibitors of collagenolytic enzymes in various cell culture fluids.