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The effects of bacterial sonicates on human keratinizing stratified squamous epithelium in vitro
Author(s) -
Kamen P. R.
Publication year - 1981
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1981.tb00981.x
Subject(s) - in vitro , epithelium , microbiology and biotechnology , stratified squamous epithelium , actinomyces , actinomyces naeslundii , biology , keratinocyte , cell culture , cell , chemistry , bacteria , biochemistry , genetics
Direct cytotoxicity of plaque is believed to play a role in the soft tissue destruction observed in periodontal disease. This property has been demonstrated in vitro on cell lines but appears to be dependent upon the target cell used and the plaque organism being tested. The purpose of this investigation was to determine the effects of bacterial products on growth of keratinized stratified squamous epithelium maintained in vitro. Morphological features of these cultures closely resembled normal epidermis. Sterile sonicates of the periodontal pathogenes Bacieroides asaccharolyticus and Actinomyces, naeslundii were diluted to equal protein concentrations and added lo keratinocyte cultures. The Bacteroides sonicate caused up to 97 % inhibition of labelled thymidine uptake of cultured keratinocytes while the peak inhibition of the Actinomyces sonicate was only 39 % of untreated controls. These results indicate that products of B.asaccharolyticus were more inhibitory to the growth of cultured keratinocytes than products of A. naes lundii. and that keratinocyte cultures make a useful model for investigation of the interactions between periodontal pathogens and differentiating epidermal cells.