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Association of oral Bacteroides with gingivitis and adult periodontitis
Author(s) -
White D.,
Mayrand D.
Publication year - 1981
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1981.tb00974.x
Subject(s) - bacteroides , microbiology and biotechnology , periodontitis , gingivitis , biology , medicine , bacteria , dentistry , genetics
The microbial flora of healthy gingival crevices and of periodontal pockets was studied to determine if a relation exists between the presence of Bacteroides asaccharolyticus and/or saccharolytic subspecies of B. melaninogenicus and the severity of the disease. Seventy‐eight samples were collected with sterile absorbent points from 40 individuals. The samples were immediately placed in reduced transport fluid. After dispersal by sonic treatment, the samples were put into an anaerobic chamber, serially diluted and plated on modified MM10 medium. After incubation, plates were examined and major bacterial types were counted. Black‐pigmented colonies were selected for further characterization. Samples from patients with severe inflammation (gingival index, GI = 3) showed a greater proportion of Gram‐negative anaerobic rods and 31.8 % of these organisms were identified as B. asaccharolyticus. This organism was absent in healthy sulci. It was recovered from mildly inflamed sites (GI = 1 or 2) but less often than B. melaninogenicus ss intermedius. B. melaninogenicus ss melaninogenicus was found only occasionally and in low numbers.