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The effect of parathyroid hormone on the numbers of nuclei in feline odontoclasts in vivo
Author(s) -
Addison W. C.
Publication year - 1980
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1980.tb00311.x
Subject(s) - cats , in vivo , endocrinology , medicine , hormone , parathyroid hormone , cytoplasm , chemistry , pathology , biology , calcium , biochemistry , microbiology and biotechnology
Odontoclasts exhibit varying numbers of nuclei. The purpose of the present study was to determine the effect of a low dose (4 USP units per 100 g. body weight) of parathyroid hormone extract (PTE) in vivo on the numbers of nuclei in whole feline odontoclasts. Odontoclasts were recovered in smear type preparations from the resorbing primary teeth of 12 kittens aged approximately 18 weeks. Four kittens had received PTE intravenously one hour prior to sacrifice, four six hours previously, and four were controls. The fresh smears were allowed to dry and subjected to dehydrogenase enzyme histochemical reactions. The unstained odontoclast nuclei contrasted with the stained cytoplasm and were counted at a magnification of × 125. The three groups had similar distribution patterns of nuclei per cell but progressive increases in the mean and median numbers of nuclei per cell were found following PTE treatment. The mean in control animals was 7.0 S.E. ± 0.12 and in the PTE 1 hour and PTE 6 hours groups was 8.0 S.E. ± 0.12 and 8.9 S.E. ± 0.12 respectively. The median values were 5.5, 6.6 and 7.3 respectively. The differences between both the control and PTE 1 hour, and between the PTE 1 hour and PTE 6 hours groups were statistically significant (p < 0.001). This rapid increase in the numbers of nuclei in odontoclasts after PTE treatment has not been reported previously.