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Improvements of in vitro cell‐material adhesion measurements
Author(s) -
Fletcher Ronald D.,
Adamiak John K.,
Labant Maryann C.,
Abertson John N.
Publication year - 1979
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1979.tb00248.x
Subject(s) - adhesion , adhesive , in vitro , cell adhesion , agar , chemistry , cell , substrate (aquarium) , agar plate , contact angle , biophysics , materials science , composite material , biology , biochemistry , bacteria , ecology , layer (electronics) , genetics
The in vitro measurement of gingival cell‐bacto agar substratum adhesive‐bonding strength (γ a ) is described as it relates to cell‐ concentration, cell‐substratum contact time and serum concentration. These three factors are critical in obtaining reprocible γ a 's for gingival cell‐test substratum interactions. Cell concentrations of less than 35 × 10 3 cells per cm 2 gave low γ a 's (70–75 ergs/cm 2 ) whereas 90–130 × 10 3 cells/cm 2 resulted in γ a 's of 109–119 ergs/cm 2 , respectively. Maximum γ a 's (123 ergs/cm 2 ) resulted after the cells had been in contact with the agar substratum for 24 hours. The adhesive‐bonding strenth after eight housrs of contact time, however, was not statistically different from the 24 housr readings and has the advantage of decreasing the test duration. Serum, and important factor for optimum adhesion measurements, produced a peak γ a at the 10 % concentration.