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Mitosis in epithelium of murine palatal mucosa in vivo and in vitro
Author(s) -
Luke D. A.
Publication year - 1979
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1979.tb00226.x
Subject(s) - mitosis , in vivo , epithelium , biology , colchicine , oral mucosa , basal (medicine) , in vitro , andrology , microbiology and biotechnology , anatomy , medicine , endocrinology , genetics , insulin
Colchicine‐arrested metapheses were accumulated at half‐hourly intervals over a period of 5 hours in the palatal epithelium of adult male mice. From the rate of entry into mitosis, which was reasonably rectilinear, the mean cell cycle time of the basal layer in vivo was found to be 80.6 h and the duration of mitosis 1.4 h. When a similar accumulation of arrested metaphases was attempted over a period of 4 hours in palatal epithelium in vitro , the rate of entry into mitosis was not rectilinear and the mitotic activity was much lower than in vivo. It is concluded that this in vitro technique is not satisfactory for determining cell cycle characteristics in oral epithelium of the living animal.