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Detachment od cells in vitro with culture fluids from stimulated human mononuclear cells
Author(s) -
Neiders Mirdza E.,
Horton John E.
Publication year - 1978
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1978.tb00192.x
Subject(s) - peripheral blood mononuclear cell , in vitro , cell culture , stimulation , in vivo , tissue culture , chemistry , microbiology and biotechnology , biology , biochemistry , endocrinology , genetics
Supernatants of cultured mononuclear cells (S‐MNL) were tested for their ability to separate cells from their substrates. Adherent L‐cells were exposed for up to 3 hr to culture fluids from MNL with and without PHA stimulation. A reproducible shearing force was then applied to the L‐cells which tests for loss of cohesiveness at the cell periphery. We found a significant increase of 3‐fold or greater in the number of detached L‐cells receiving stimulated S‐MNL when compared with culture fluids from unstimulated MNL. The detachment‐enhancing activity was present in culture fluids within 24 hours following stimulation. Examination of MNL culture fluids revealed no differences in the quantities of lysosomal enzymes to explain the increased cell detachment. We suggest the detachment of cells seen in vitro by products released from mononuclear cells may provide an explanation for the in vivo ability of these cells to infiltrate tissue.