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Studies in plaque pathogenicity
Author(s) -
Fine D. H.,
Tabak L.,
Salkind A.,
Oshrain H.
Publication year - 1978
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1978.tb00161.x
Subject(s) - limulus , trichloroacetic acid , lysis , lysozyme , limulus amebocyte lysate , dental plaque , phenol extraction , nucleic acid , extraction (chemistry) , microbiology and biotechnology , virus quantification , chemistry , lysis buffer , chromatography , biology , biochemistry , rna , lipopolysaccharide , virology , immunology , virus , paleontology , gene
The purpose of this study was to define a technique for the positive identification of endotoxin in heterogenous dental plaque samples. An extraction technique, which includesan acotone wash and trichloroacetic acid precipitation followed by pphenol‐water extraction was developed to reduce the level of both proteins and nucleic acids in individual plaque samples. Ten subgingival samples of adherent and loosely adherent treated in this manner retained limulus lysate almost exclusively in the phenol phase; ten supragingival samples retained activity in the phenol and water phases. further treatment of all of these samples with ribonuclease A and T 1 and with lysozyme did not alter assay results, suggesting that the limulus lysate activity detected was due to Gram‐negative Bacterial cell wall fractions. this technique may be useful in determining endotoxin activity in plaque samples and ultimately, in evaluation the effectiveness of chemotherapeutic agents in reduction of plaque endotoxin.