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Monocyte chemotactic activity in human dental plaque
Author(s) -
Helldén Leif
Publication year - 1977
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1977.tb00106.x
Subject(s) - monocyte , chemotaxis , dental plaque , chemistry , sephadex , in vitro , starch , peritoneal cavity , hydrolysis , microbiology and biotechnology , biochemistry , dentistry , biology , immunology , medicine , enzyme , anatomy , receptor
The monocyte chemotactic activity elaborated by soluble dental plaque material and by various fractions of the plaque material was studied in vitro with the modified Boyden chamber technique. Monocytes were harvested from the peritoneal cavity of rabbits after intraperitoneal injection of a 1 % suspension of hydrolysed starch. The plaque material was separated in Sephadex gel columns (G‐25 and G‐20Q). Three fractions differing from each other in molecular size were selected from the protein peaks in the chromatograms. The results showed that dental plaque material contains factors with a chemotactic effect on monocytes. Examination of various fractions of dental plaque material revealed that the major part of the monocyte chemotactic activity resided in a fraction with an appoximate molecular size of 10,000–70,000.