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The role of complement in initial gingivitis
Author(s) -
Kahnberg KE.,
Lindhe J.,
Attström R.
Publication year - 1976
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1976.tb00081.x
Subject(s) - beagle , gingivitis , junctional epithelium , gingival margin , epithelium , pathology , inflammation , phagocytosis , electron microscope , chemistry , medicine , biology , anatomy , immunology , dentistry , physics , optics
The effects of decomplementation by Cobra Venom Factor (CVF) on cell migration and vascular permeability in experimentally induced initial gingival inflammation in the dog have been determined. Gingivitis was provoked in four Beagle dogs with normal gingiva during an eight hour period by topical application of an extract of human plaque. In each dog, the applications were performed on one side of the jaws before, and on the contralateral side after decomplementation. Following six and a half hours of plaque extract application, colloidal carbon was injected intravenously in one of the hind legs. At the end of the application period biopsies were sampled, the tissue sections were embedded in Epon and semi thin sections (2–3 μm) were prepared and examined in the light microscope regarding (1) amount of carbon containing vascular units subjacent to the junctional epithelium (2) number of neutrophils and mononudear leukocytes within the junctional epithelium. The present experiment indicates that (1) plaque extract contains substances which, when applied onto the margin of normal gingiva, mediate attraction of leukocytes and alterations of the function of the dento‐gingival vasculature i.e. induce symptoms of initial gingivitis. (2) in the decotnplemented animals the plaque components displayed a) a reduced capacity to influence vascular function but b) an almost unaltered leukocyte attracting potential with regard to neutrophils.