Premium
In vitro cultivation of adult human gingiva
Author(s) -
Mlinek A.,
Buchner A.
Publication year - 1975
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1975.tb00010.x
Subject(s) - explant culture , in vitro , epithelium , stratified squamous epithelium , dentistry , tissue culture , mitosis , pathology , biology , anatomy , andrology , medicine , microbiology and biotechnology , biochemistry
The in vitro behavior of human gingiva was studied using a simple tissue culture technique. Gingival explants from adult patients undergoing periodontal surgery were cultivated in three different media for periods up to three weeks. It was demonstrated that primary epithelial cell cultures can be obtained under simple and predictable conditions. The migrating cells were compact and uniform in shape and resembled the spinous cell layer of a squamous stratified epithelium. Mitotic activity was regularly observed all over the outgrowth, which continued to grow throughout the entire experimental period.