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Effect of periodontal dressings on fibroblasts in vitro
Author(s) -
Hildebrand Carole N.,
Renzis Frank A.
Publication year - 1974
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1974.tb00662.x
Subject(s) - eugenol , stain , in vitro , cytotoxicity , chemistry , monolayer , vital stain , dentistry , periodontal fiber , staining , medicine , pathology , biochemistry , organic chemistry
This study was designed to evaluate the effect of four commercially available dressings (Coe, PPC‐Eugcnol, PPC‐Non Eugenol, and Ward's Wondr Pak) on a monolayer of cells, employing the cell culture assay plate assembly. Monolayers of L929 fibroblasts were grown out on the cell culture assay plates and stained with a vital stain, .01 % Neutral Red, 8 mm discs of prepared periodontal dressings, as well as sterile filter paper and high density polyethylene discs, which served as controls, were placed on the monolayer. Observations were made at 1, 2, 6, 8 and 24 hour intervals, and measurements of the toxic zones were recorded. Results indicate that all periodontal dressings tested in this system demonstrated definite cytotoxicity. Both macroscopically and microscopically, the first signs of cell damage and/or death were observed adjacent to Ward's Wondr Pak and PPC ‐Non Eugenol dressings. Although these two dressings produced the earliest toxic signs, after 24 hours Coe‐Pak produced the most extensive zones of destruction, while PPC ‐Eugenol appeared to be the least toxic.