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Bone resorption in vitro induced by products of human gingival cells in culture
Author(s) -
Jacobsen N.,
Goldhaber P.
Publication year - 1973
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1973.tb01755.x
Subject(s) - resorption , bone resorption , chemistry , cell culture , in vitro , trypsin , epithelium , periodontal fiber , connective tissue , dentistry , endocrinology , pathology , biology , medicine , biochemistry , enzyme , genetics
Resorption of bone in tissue culture has previously proved to be initiated by a combined cultivation with gingival tissues. In the present experiments bone resorption stimulating activity (BRS‐activity) was recovered in sonicates of fibroblastlike cells from human gingiva. BRS‐activity was also recovered from a transformed cell line previously reported by Smulow and Glickman (1966) to be derived from human gingival epithelium. The maximum activity was comparable to that of parathyroid extract. The BRS‐activity of the Smulow‐Glickman epithelial cell line was present both in the culture medium, in salt solution after incubation with the cells, and in cell sonicate. There was a positive dose response between the concentration of cell products and bone resorption up to a level, after which inhibition of the bone resorption occurred. The activity was stable at 56°C, resistant to trypsin, but labile at 100°C and partly dialyzable. He‐La cells were negative as regards BRS‐activity.