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Fructoaldolase activity in non‐inflamed attached gingiva of rhesus monkeys
Author(s) -
Bergquist J. J.,
Nuki K.
Publication year - 1973
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1973.tb00761.x
Subject(s) - connective tissue , gingivectomy , epithelium , epithelial tissue , pathology , mucous membrane , stratum spinosum , chemistry , basal (medicine) , granular layer , granulation tissue , anatomy , biology , medicine , dentistry , wound healing , stratum corneum , endocrinology , immunology , insulin , cerebellum
Ultramicrochemical methods were used to assay fructoaldolase activity under optimum substrate mixture concentrations in clinically non‐inflamed attached gingival tissue of Rhesus monkeys. Precautions were taken to assure the clinical non‐inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 14 to 240 nanograms (10 −9 grams). Optimum assay conditions for the microassay of freeze dried fragments were determined in fresh gingival homo‐genates. Quantitative results for the various strata of epithelium, connective tissue and whole epithelium were calculated. The mean fructoaldolase activity was reported in micromoles of converted substrate per gram of dry tissue weight per minute at 37°C: surface layer 4·04, granular layer 15·57, spinous layer 23·58, basal layer 28·10, connective tissue 12·99 and whole epithelium 26·75. These results are higher than fructoaldolase activity reported in the literature for other epithelial tissue, such as skin of the parietal region of the scalp and the plantar surface of the foot. However, the results of the present investigation are compatible with the activity assayed for mucous membrane of the lip, a tissue less commonly involved in chronic inflammatory processes than is the gingival tissue assayed in this study.