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Hexokinase activity in non‐inflamed attached gingiva of rhesus monkeys
Author(s) -
Bergquist J. J.,
Nuki K.
Publication year - 1973
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1973.tb00760.x
Subject(s) - connective tissue , epithelium , gingivectomy , pathology , hexokinase , epithelial tissue , chemistry , mucous membrane , basal (medicine) , granular layer , granulation tissue , anatomy , biology , medicine , wound healing , dentistry , biochemistry , endocrinology , diabetes mellitus , immunology , enzyme , glycolysis , cerebellum
Ultramicrochemical methods were used to assay hexokinase activity under optimum substrate mixture concentrations in clinically non‐inflamed attached gingival tissue of Rhesus monkeys. Precautions were taken to assure the clinical non‐inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 14 to 240 nanograms (10 −9 grams). Optimum assay conditions were determined in fresh gingival homogenates. Quantitative results for the various strata of epithelium, connective tissue and whole epithelium were calculated. The mean hexokinase activity was reported in micromoles of converted substrate per gram of dry tissue weight per minute at 37°C: surface layer 22.4. granular layer 22.6, spinous layer 18.9, basal layer 15.9, connective tissue 16.6 and whole epithelium 18.0. These results are compatible, for the most part, with hexokinase activity assayed in other epithelial tissue, such as skin of the parietal region of the scalp, plantar surface of the foot and mucous membrane from the lip, all of which are less commonly involved in chronic inflammatory processes than is gingival tissue.