Premium
Malic dehydrogenase in non‐inflamed attached gingiva of dogs
Author(s) -
Bergquist J. J.
Publication year - 1972
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1972.tb01110.x
Subject(s) - connective tissue , gingivectomy , epithelium , pathology , basal (medicine) , chemistry , biology , anatomy , medicine , dentistry , endocrinology , insulin
Ultramicrochemical methods were used to assay malic dehydrogenase (M.D.H.) activity in non‐ inflamed attached gingiva of dogs. Precautions were taken to assure the clinical and histological non‐inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 12 to 240 manograms. Quantitative results for various strata of epithelium, connective tissue and whole epithelium were calculated. The mean M.D.H. activities in micromoles of substrate converted, per gram of dry tissue weight per minute at 37° C. were: surface layer, 29; granular layer, 222; spinous layer, 329; basal layer, 598; connective tissue, 71; and whole epithelium, 337. Differences in aerobic metabolism between the present findings in dogs and those reported for rat oral mucosa may in part be due to the type of keratinization in the two species. These differences in M.D.H. activity in dog and rat tissue are compatible with previosuly reported results of investigations of the oxygen consumption of these tissues.