Premium
A study of the elimination of chlorhexidine from tthe oral cavity using a new spactrophotometric method
Author(s) -
Jensen Jørn Erik,
Christensen Flemming
Publication year - 1971
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1971.tb00622.x
Subject(s) - chlorhexidine , saliva , dentistry , chemistry , medicine , biochemistry
A method for the determination of chlorhexidine in saliva is described. The mean recovery rates observed with chlorhexidine digluconate added to saliva in the ranges 5–25,μg/ml and 25–200 μg/ml where 103.6 % and 102.8 % respectively. The lower limit of the method is in the vicinity of 5 μg of chlorhexidine digluconate. The accuracy was found to be ± 3 % and ± 1 % when the concentration of the samples were in the ranges 5–25 μg/ml and 25–200 μg/ml respectively. The method is not specific for chlorhexidine and the amount of interfering material in saliva was found to be equivalent to 3.45 ± 0.54 μg/ml of chlorhexidine digluconate. The rate of elimination of chlorhexidine digluconate from the mouth after one mouthrinse with 20 mg followed approximately the equation describing a process of the first order. The mean values of the half‐times (t/2) and the initial concentrations (C max ) was found to be 63 ± 11 min and 153 + 27 μg/ml respectively. The concentration of chlorhexidine digluconate was higher than the proposed minimal inhibitory concentration for oral streptococci of 5 μg/ml for approximately 5 hours. The significance of this in relation to the clinical effect of mouthrinsing with chlorhexidine is discussed. The amount of chlorhexidine digluconate swallowed after one mouthrinse was estimated to be approximately 4 mg corresponding to 20 % of the dose.