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On the hydroxyproline‐Containing components in the gingival exudate
Author(s) -
Paunio Keijo
Publication year - 1971
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1971.tb00597.x
Subject(s) - exudate , hydroxyproline , chemistry , connective tissue , chromatography , sephadex , gingival sulcus , gel permeation chromatography , hexosamines , dentistry , biochemistry , enzyme , pathology , medicine , organic chemistry , glucosamine , polymer
The aim of the study was to determine the presence of hydroxyproline‐containing components in the gingival exudate. The material was collected with filter paper strips from more than 100 individuals. Two pools were made: one for gel permeation chromatography, on Sephadex G‐200 columns, and one for dialysis, against tap water. Single strips containing gingival exudate from one single sulcus were also analyzed. The analysis of the individual strips was made directly from the supernatant fluid. The results from the pooled samples show that about 50 % of the hydroxyproline‐containing components were fractionated into two peaks in column chromatography. The hexosamines were also fractionated into the same peaks. The hydroxyproline containing components in the gingival exudate may arise from blood serum leaking into the crevice or from gingival collagen degraded by proteolytic enzymes originating from the gingival tissue itself or from plaque. These can be considered as a sign of collagen metabolism and/or of a plaque enzyme action on the connective tissue in the gingiva.