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Penetration pathway of a topically applied foreign protein into rat gingiva
Author(s) -
McDougall W. A.
Publication year - 1971
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1971.tb00594.x
Subject(s) - horseradish peroxidase , connective tissue , chemistry , staining , pathology , anatomy , microbiology and biotechnology , biology , biochemistry , medicine , enzyme
Topically applied horseradish peroxidase (HRPO) failed to penetrate through keratinized rat gingiva. It rapidly entered the intercellular spaces of the non‐keratinized epithelial attachment and within 10 minutes it spread as far as the attachment's apical limit. Widening of the intercellular spaces occurred. Spread into the underlying connective tissue was associated with dilation of venules and, after 30 minutes, emigration of neutrophils into the epithelial attachment. Macrophages and to a lesser extent, fibroblasts showed uptake of HRPO into vesicles and lysosomes. Some macrophages containing HRPO were located close to the crest of the alveolus. A few cells of theepithelial attachment also showed the presence of HRPO in lysosomes.