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The applicability of a special freeze‐sectioning technique in enzyme histochemical studies of molar tissues in the mouse
Author(s) -
Heyden Guy
Publication year - 1970
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1970.tb00731.x
Subject(s) - epithelium , molar , connective tissue , succinate dehydrogenase , chemistry , pathology , enzyme , oxidative enzyme , biology , anatomy , biochemistry , medicine , paleontology
The freeze‐sectioning technique of Ullberg (1954, 1958) was tested for enzyme histochemical studies of molar eruption in the mouse when cutting the tissues in a cryostat. The method was found applicable for the purposes intended, not only in lactate and malate dehydrogenase recording but also, and especially, in those for glucose‐6‐phosphate dehydrogenase and adenosine‐triphosphatase activities in calcified tissues. The advantages and disadvantages of the technique, were discussed as compared with the difficulties of EDTA‐demineralization. Marked oxidative and hydrolytic enzyme reactions were observed in the reduced dental epithelium even in progressed stages of molar crown eruption. Hard‐tissue forming cells revealed cytoplasmic ATPase activity as well as endothelial cells and connective tissue regions beneath heavily keratinized and unstained oral epithelium. The cervical migration of the oral epithelium, after the fusion with the reduced dental epithelium, could be discerned by the different distributions of enzyme histochemical reactions.