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Observations on vascular proliferation in a granulation tissue
Author(s) -
Lindhe Jan,
Brånemak PI
Publication year - 1970
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1970.tb00729.x
Subject(s) - granulation tissue , degranulation , anatomy , infiltration (hvac) , pathology , granulation , chemistry , wound healing , biology , medicine , materials science , immunology , biochemistry , receptor , composite material
The present study was performed in order to study the progressive growth of vessels in a regenerating tissue and to analylze the tissue events occurring outside and inside the mature micro‐vessels immediately before sprout formation. Twenty, 10–20 months old female rabbits, weighing 2–3 kilos each were used. A modified Sandison's ear chamber was installed in each animal. Healing of a tissue defect was studied in a modified Leitz intravital microscope. Immediately after installation of the ear chamber, the center of the wound was practically devoid of cells. The venules of the bordering tissue showed signs of an acute inflammation. During the first hours there was an obvious leakage from the venules of the bordering tissue of an intravenously injected protein bound dye. Some hours later granular cells started to emigrate from the adjacent pre‐existing venules into the defect space. Six to eight hours after surgery corpuscular and plasma flow was normal in all vessels in the region of the ear chamber. During the next 35–40 hours the protein network became denser. By the end of the second day most of the granular cells of the defect had lost their amoeboid properties. The nucleus and the granules of these cells became denser and more prominent. By 48–72 hours several of the rigid granular cells disintegrated and dispersed their cytoplasmic granules into the defect. A few hours after degranulation the corpuscular flow rate of the bordering vessels increased. Erythrocytes and later granulocytes penetrated the venular walls and entered the extra‐vascular space. In the immediate vicinity of the vertices of the preformed venules, strings of red and white cells moved back and forth apparently creating well defined tubes bordered by a fibrillar structure. The cell movements seemed to open up and elongate the tubes. Between the moving cells and aggregated cells of the defect a layer of thin transparent cells could eventually be detected. In this situation there were also optically definable openings in the walls of the borderline venules and true communicationsestablished between the preexisting vessels and the newly formed.