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The uptake of H 3 ‐estradiol by the oral tissues of rats
Author(s) -
Formicola Allan J.,
Weatherford Thomas,
Grupe Harold
Publication year - 1970
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1970.tb00728.x
Subject(s) - estrogen , ovariectomized rat , uterus , endocrinology , medicine , chemistry , kidney , oral mucosa , pathology
This study was designed to determine whether H 3 ‐estradiol would localize in the oral tissues of rats. Twenty ovariectomized rats were injected subcutaneously with H 3 ‐estradiol. After injection, oral tissue including gingivae and mucosa, submandibular gland, liver, kidney, uterus and skin were dissected, weighed and homogenized. The tissues were assayed for H 3 ‐estradiol and 5 ml aliquots added to liquid scintillation fluid. The samples were read on a Beckman Liquid Scintillator. A series of experiments using the above technique demonstrated the following: (1) H 3 ‐estradiol concentrated in the oral extract when a dose of 0.136 μg/100 gm body weight or greater was given. (2) The assay of oral extract contained H 3 ‐estradiol as evidenced by chromatography. (3) One hour after injection the oral extract had the highest concentration of H 3 ‐estradiol (212 CPM/mg of tissue) while the uterus remained low (47.7 CPM/mg) in uptake. Four hours after injection there was a statistically significant increase of H 3 ‐estradiol in the uterus (118.5 CPM/mg but not a significant decrease of H 3 ‐estradiol in the oral extract (131.8 CPM/mg). (4) Estrogen (Progynon) * priming for 7 days reduced the uptake of H 3 ‐estradiol in the oral extract. Since H 3 ‐estradiol concentrates in the oral extract, it is suggested that estrogen can influence the gingival tissues on a local cellular level. The fact that estrogen priming reduced the uptake of H 3 ‐estradiol in the oral extract indicates that there may be some type of binding site in the gingivae and mucosa.