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A study of the effects of gingival plaque extracts on cells cultured in vitro
Author(s) -
Baboolal R.,
Mlinek A.,
Powell R.N.
Publication year - 1970
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1970.tb00724.x
Subject(s) - lysis , in vitro , monosaccharide , phenol , cytoplasm , biochemistry , chemistry , electrophoresis , cell growth , amino acid , cell , microbiology and biotechnology , biology , organic chemistry
The effects of soluble and phenol extracted fractions of human dental plaque obtained from the gingival crevice on the growth and morphology of Baby Syrian Hamster cells (BHK21) were investigated. The results indicated that cells grown in the presence of both fractions showed considerable degenerative morphological changes involving lysis of the cytoplasm, deterioration of the nuclei and intra nuclear structures, when compared with control cultures. Both fractions produced marked inhibition on the growth cycle resulting in significant decrease in cell numbers. However, there were no significant differences between the soluble and phenol extracted fractions with regard to their ability to inhibit cell growth. Preliminary analyses of the fractions revealed that the soluble contained 15% protein and several reducing monosaccharides and two amino sugars. In the phenol extracted portions no proteins were detected. Electrophoretic studies indicated that both fractions were composed of more than one molecular species.