Endosteal bone formation following vascular obstruction

Partial occlusion of the periodontal microvasculature was achieved by introducing black carbonized microspheres unilaterally into the external carotid artery of squirrel monkeys. The contralateral side of the animal served as its own control. One week following the vascular obstruction, tritiated 2‐glycine was injected intraperitoneally to label newly formed endosteal bone. All animals were sacrificed four weeks following the injection of tritiated glycine. The jaws were removed, hemi‐sected, fixed, decalcified, and sectioned bucco‐lingually through the interdental areas (cols). Autoradiography was performed on tissue sections selected from the posterior col areas of the experimental and control sides. Measurements were made from sections of interdental labelled and unlabelled endosteal bone surfaces. Less endosteal bone formation was evident on the experimental side following the vascular insult.