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Effects of demineralization and parathyroid hormone on the availability of bone collagen to degradation by collagenase
Author(s) -
Stern B.,
Golub L.,
Goldhaber P.
Publication year - 1970
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1970.tb00702.x
Subject(s) - calvaria , collagenase , parathyroid hormone , bone resorption , chemistry , demineralization , resorption , type i collagen , medicine , endocrinology , bone mineral , in vitro , biochemistry , dentistry , osteoporosis , calcium , biology , enzyme , enamel paint , organic chemistry
Calvaria were exposed to collagenase in vitro before and after demineralization to determine the effect of the mineral phase on the availability of the collagen matrix to collagenolytic degradation. Other calvaria were cultured, in the presence or absence of parathyroid hormone, in a system that supports active bone resorption but not bone formation. The residual calvaria were then treated with collagenase as described above to study the availability and susceptibility of the remaining bone collagen to enzymatic attack. The mineral phase of the calvaria was found to protect collagen in bone from degradation by collagenase. Removal of the mineral from these bones rendered the remaining pool of collagen available to collagenolytic attack. Both unmineralized and mineralized pools of collagen in the calvaria were degraded during bone resorption in culture and this process was stimulated by parathyroid hormone. Since the demineralized cultured calvaria were degraded by collagenase in vitro to a greater extent than the demineralized uncultured calvaria, it would appear that the collagen matrix, in addition to being made available to collagenolytic attack through the loss of mineral, was altered structurally during bone resorption such that it became more susceptible to enzymatic degradation.