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Formation of dentin and bone matrix in magnesium‐deficient rats
Author(s) -
Trowbridge Henry O.,
Seltzer Judith L.
Publication year - 1967
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1967.tb01882.x
Subject(s) - sulfation , dentin , chemistry , alkaline phosphatase , magnesium , polysaccharide , hydroxyproline , biochemistry , magnesium deficiency (plants) , matrix (chemical analysis) , endocrinology , medicine , enzyme , chromatography , biology , dentistry , organic chemistry
Collagen formation and the sulfation of protein‐polysaccharides in the organic matrix of bone and dentin was studied in young, magnesium‐deficient rats of the Long‐Evans strain. Autoradiography was employed to assess the incorporation of tritiated proline into collagen, and 35 S sulfate was utilized to study the sulfation of protein‐polysaccharides. Our observations on the uptake of these radioactive precursors indicate that a deficiency of magnesium interferes both with collagen formation and the sulfation of glycosaminoglycuronoglycans in the organic matrices of bone and dentin. Alkaline phosphatase activity, determined in serum samples and observed histochemically in calcifying tissues, was depressed in the magnesium‐deficient animals as compared with controls. The localization of alkaline phosphatase activity in bone and dentin forming tissues from experimental rats was found to be similar to that of the controls, but the intensity of the staining reaction was reduced in the tissues from the magnesium‐deficient animals. Suppression of enzyme systems known to be activated by magnesium and related to protein and protein‐polysaccharide biosynthesis would, in part, explain the results of this experiment.