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Presence of histologically demonstrable bound lipid in gingival connective tissue and loss during collagenolysis
Author(s) -
Melcher A. H.
Publication year - 1966
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1966.tb01867.x
Subject(s) - connective tissue , acetone , chemistry , staining , sudan black b , extracellular , pathology , biology , anatomy , biochemistry , medicine
Cold microtome sections of healthy connective tissue and connective tissue being resorbed pathologically in chronic inflammation and physiologically in the path of erupting teeth from adolescent and mature Macaca irus monkeys were examined for the presence of Sudanophilic bound lipid. Healthy connective tissue of mature gingiva, like a number of other connective tissues, was found to contain a spectrum of varyingly bound extracellular Sudanophilic lipids stainable by Sudan black/acetone, but not by Sudan black/70% alcohol, without prior extraction, or after extraction in acetone or acidified chloroform: methanol. These bound lipids were soluble in lipid solvents. The intense Sudanophilia of the lipid stainable after extraction in acidified chloroform: methanol was absent from areas where collagenolysis had occurred, suggesting that the lipid had been released. There was no difference in lipid staining between connective tissues being resorbed physiologically and pathologically. Deposits of extracellular lipid stained by Sudan black/acetone were present in healthy mature connective tissue and in areas adjacent to collagen destruction. Lipid stained in the former situation is thought to be involved in remodelling of the connective tissue; that in the latter, possibly to comprise both lipid unmasked in collagenolysis and lipid being incorporated into connective tissue during reactive fibro‐genesis.